Thermodynamically balanced inside-out (TBIO) PCR-based gene synthesis: a novel method of primer design for high-fidelity assembly of longer gene sequences

A novel thermodynamically-balanced inside-out (TBIO) method of primer design was developed and compared with a thermodynamically-balanced conventional (TBC) method of primer design for PCR-based gene synthesis of codon-optimized gene sequences for the human protein kinase B-2 (PKB2; 1494 bp), p70 ri...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Gao, Xinxin, Yo, Peggy, Keith, Andrew, Ragan, Timothy J., Harris, Thomas K.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 2003
Assuntos:	NAR Methods Online
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC275580/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14602936/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng143
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