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Multiplex quantitative PCR using self-quenched primers labeled with a single fluorophore

Multiplex quantitative PCR based on novel design of fluorescent primers is described. Fluorogenic primers are labeled with a single fluorophore on a base close to the 3′ end with no quencher required. A tail of 5–7 nt is added to the 5′ end of the primer to form a blunt-end hairpin when the primer i...

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Bibliografiska uppgifter
Huvudupphovsmän: Nazarenko, Irina, Lowe, Brian, Darfler, Marlene, Ikonomi, Pranvera, Schuster, David, Rashtchian, Ayoub
Materialtyp: Artigo
Språk:Inglês
Publicerad: Oxford University Press 2002
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Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC113860/
https://ncbi.nlm.nih.gov/pubmed/11972352
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