Multiplex quantitative PCR using self-quenched primers labeled with a single fluorophore

Multiplex quantitative PCR based on novel design of fluorescent primers is described. Fluorogenic primers are labeled with a single fluorophore on a base close to the 3′ end with no quencher required. A tail of 5–7 nt is added to the 5′ end of the primer to form a blunt-end hairpin when the primer i...

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Publié dans:	Nucleic Acids Res
Auteurs principaux:	Nazarenko, Irina, Lowe, Brian, Darfler, Marlene, Ikonomi, Pranvera, Schuster, David, Rashtchian, Ayoub
Format:	Artigo
Langue:	Inglês
Publié:	Oxford University Press 2002
Sujets:	NAR Methods Online
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC113860/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11972352/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/30.9.e37
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