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Biologically-generated primer for PCR: PCR primer of unknown sequence.

We describe a method for producing specific PCR primers directly from PCR product, bypassing the usual need to know the primer sequence. Lack of abundance of primers derived from a PCR product is compensated for by the incorporation of an arbitrary 5'TAG sequence which acts as a surrogate templ...

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Bibliografski detalji
Glavni autori: Bindon, C, Martindale, J, Mitchell, C
Format: Artigo
Jezik:Inglês
Izdano: 1998
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC147670/
https://ncbi.nlm.nih.gov/pubmed/9628934
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