Direct detection of Brucella spp. in raw milk by PCR and reverse hybridization with 16S-23S rRNA spacer probes.

The 16S-23S rRNA spacer regions of Brucella abortus, B. melitensis, and B. suis were cloned and subcloned after PCR amplification. Sequence analysis of the inserts revealed a spacer of about 800 bp with very high ( > 99%) homology among the three species examined. Two genus-specific primer pairs, BR...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Rijpens, N P, Jannes, G, Van Asbroeck, M, Rossau, R, Herman, L M
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1996
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167942/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8633866/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.62.5.1683-1688.1996
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