Biologically-generated primer for PCR: PCR primer of unknown sequence.
We describe a method for producing specific PCR primers directly from PCR product, bypassing the usual need to know the primer sequence. Lack of abundance of primers derived from a PCR product is compensated for by the incorporation of an arbitrary 5'TAG sequence which acts as a surrogate template t...
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| Publicat a: | Nucleic Acids Res |
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| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1998
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147670/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9628934/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.13.3305 |
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