Multiplex quantitative PCR using self-quenched primers labeled with a single fluorophore
Multiplex quantitative PCR based on novel design of fluorescent primers is described. Fluorogenic primers are labeled with a single fluorophore on a base close to the 3′ end with no quencher required. A tail of 5–7 nt is added to the 5′ end of the primer to form a blunt-end hairpin when the primer i...
שמור ב:
| הוצא לאור ב: | Nucleic Acids Res |
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| Principais autores: | , , , , , |
| פורמט: | Artigo |
| שפה: | Inglês |
| יצא לאור: |
Oxford University Press
2002
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| נושאים: | |
| גישה מקוונת: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC113860/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11972352/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/30.9.e37 |
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