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Efficient labeling of fission yeast Schizosaccharomyces pombe with thymidine and BUdR
In this paper we report the construction of a Schizosaccharomyces pombe strain that facilitates analysis of replicating DNA. The strain co-expresses the Herpes simplex virus thymidine kinase gene (hsv-tk) and a human equilibrative nucleoside transporter (hENT1). The double integrant efficiently inco...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2003
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC275491/ https://ncbi.nlm.nih.gov/pubmed/14576334 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gng134 |
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