Efficient labeling of fission yeast Schizosaccharomyces pombe with thymidine and BUdR

In this paper we report the construction of a Schizosaccharomyces pombe strain that facilitates analysis of replicating DNA. The strain co-expresses the Herpes simplex virus thymidine kinase gene (hsv-tk) and a human equilibrative nucleoside transporter (hENT1). The double integrant efficiently inco...

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Bibliographic Details
Main Authors: Hodson, Jeffrey A., Bailis, Julie M., Forsburg, Susan L.
Format: Artigo
Language:Inglês
Published: Oxford University Press 2003
Subjects:
NAR Methods Online
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC275491/
https://ncbi.nlm.nih.gov/pubmed/14576334
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gng134
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