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Efficient labeling of fission yeast Schizosaccharomyces pombe with thymidine and BUdR

In this paper we report the construction of a Schizosaccharomyces pombe strain that facilitates analysis of replicating DNA. The strain co-expresses the Herpes simplex virus thymidine kinase gene (hsv-tk) and a human equilibrative nucleoside transporter (hENT1). The double integrant efficiently inco...

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Bibliografiske detaljer
Udgivet i:Nucleic Acids Res
Principais autores: Hodson, Jeffrey A., Bailis, Julie M., Forsburg, Susan L.
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2003
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC275491/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14576334/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng134
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