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Fast Folding of an RNA Tetraloop on a Rugged Energy Landscape Detected by a Stacking-Sensitive Probe

We investigate the microsecond-timescale kinetics of the RNA hairpin ga(∗)cUUCGguc. The fluorescent nucleotide 2-aminopurine (a(∗)) reports mainly on base stacking. Ten kinetic traces and the temperature denaturation curve are globally fitted to four-state models of the free-energy surface. In the b...

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Detalhes bibliográficos
Main Authors: Sarkar, Krishnarjun, Meister, Konrad, Sethi, Anurag, Gruebele, Martin
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2749769/
https://ncbi.nlm.nih.gov/pubmed/19720030
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2009.06.035
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