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Fast Folding of an RNA Tetraloop on a Rugged Energy Landscape Detected by a Stacking-Sensitive Probe
We investigate the microsecond-timescale kinetics of the RNA hairpin ga(∗)cUUCGguc. The fluorescent nucleotide 2-aminopurine (a(∗)) reports mainly on base stacking. Ten kinetic traces and the temperature denaturation curve are globally fitted to four-state models of the free-energy surface. In the b...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The Biophysical Society
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2749769/ https://ncbi.nlm.nih.gov/pubmed/19720030 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2009.06.035 |
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