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Structural basis for exquisite specificity of affinity clamps, synthetic binding proteins generated through directed domain-interface evolution
We have recently established a new protein-engineering strategy termed “directed domain-interface evolution” that generates a binding site by linking two protein domains and then optimizing the interface between them. Employing this strategy, we have generated synthetic two-domain “affinity clamps”...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2748140/ https://ncbi.nlm.nih.gov/pubmed/19646997 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2009.07.067 |
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