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Oligonucleotide-based assays for integrase activity

Oligonucleotide assays have been invaluable for elucidation of the molecular mechanisms of retroviral integrases. A suite of rapid and sensitive fluorescence assays to measure the DNA binding, processing, and joining activities of integrase (IN) is described here. The assays are especially useful fo...

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Bibliographic Details
Main Authors: Merkel, George, Andrake, Mark D., Ramcharan, Joseph, Skalka, Anna Marie
Format: Artigo
Language:Inglês
Published: 2008
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2743288/
https://ncbi.nlm.nih.gov/pubmed/19010419
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2008.10.024
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