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Oligonucleotide-based assays for integrase activity

Oligonucleotide assays have been invaluable for elucidation of the molecular mechanisms of retroviral integrases. A suite of rapid and sensitive fluorescence assays to measure the DNA binding, processing, and joining activities of integrase (IN) is described here. The assays are especially useful fo...

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Autors principals: Merkel, George, Andrake, Mark D., Ramcharan, Joseph, Skalka, Anna Marie
Format: Artigo
Idioma:Inglês
Publicat: 2008
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2743288/
https://ncbi.nlm.nih.gov/pubmed/19010419
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2008.10.024
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