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A General and Efficient Method for the Site-Specific Dual-Labeling of Proteins for Single Molecule FRET
[Image: see text] A general strategy for the site-specific dual-labeling of proteins for single-molecule fluorescence resonance energy transfer (smFRET) is presented. A genetically encoded unnatural ketone amino acid was labeled with a hydroxylamine-containing fluorophore with high yield (>95%) a...
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| Auteurs principaux: | , , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2008
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2743202/ https://ncbi.nlm.nih.gov/pubmed/19108697 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja807430h |
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