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Dual Unnatural Amino Acid Incorporation and Click-Chemistry Labeling to Enable Single-Molecule FRET Studies of p97 Folding
Many cellular functions are critically dependent on the folding of complex multimeric proteins, such as p97, a hexameric multi-domain AAA+ chaperone. Given p97’s complex architecture, single-molecule Förster Resonance Energy Transfer (smFRET) would be a powerful tool for studying folding while avoid...
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| Publicado no: | Chembiochem |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4904727/ https://ncbi.nlm.nih.gov/pubmed/27115850 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.201500695 |
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