Design and application of a versatile expression vector for RNAi in mammalian cells

Here we report a versatile mammalian expression vector called pRIGHT11 for production of small interfering RNA (siRNA) in cells. This vector uses opposing eukaryotic RNA polymerase III promoters U6 and H1 to drive the expression of short siRNA. We have demonstrated the effectiveness of pRIGHT11-gene...

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Bibliografske podrobnosti
Main Authors: Lin, Xia, Feng, Xin-Hua
Format: Artigo
Jezik:Inglês
Izdano: Library Publishing Media 2005
Teme:
New Methods and Technologies
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2737199/
https://ncbi.nlm.nih.gov/pubmed/19771203
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