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Optimized Bacterial Expression and Purification of the c-Src Catalytic Domain for Solution NMR Studies

Progression of a host of human cancers is associated with elevated levels of expression and catalytic activity of the Src family of tyrosine kinases (SFKs), making them key therapeutic targets. Even with the availability of multiple crystal structures of active and inactive forms of the SFK catalyti...

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Detalhes bibliográficos
Main Authors: Piserchio, Andrea, Ghose, Ranajeet, Cowburn, David
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2735562/
https://ncbi.nlm.nih.gov/pubmed/19399371
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10858-009-9318-y
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