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Determination of the folding of proteins as a function of denaturants, osmolytes or ligands using circular dichroism
Circular dichroism (CD) is an excellent tool for examining the interactions and stability of proteins. This protocol covers methods to obtain and analyze circular dichroism spectra to measure changes in the folding of proteins as a function of denaturants, osmolytes or ligands. Applications include...
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2006
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2728349/ https://ncbi.nlm.nih.gov/pubmed/17406529 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2006.229 |
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