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Determination of the folding of proteins as a function of denaturants, osmolytes or ligands using circular dichroism

Circular dichroism (CD) is an excellent tool for examining the interactions and stability of proteins. This protocol covers methods to obtain and analyze circular dichroism spectra to measure changes in the folding of proteins as a function of denaturants, osmolytes or ligands. Applications include...

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Autor principal: Greenfield, Norma J.
Format: Artigo
Idioma:Inglês
Publicat: 2006
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2728349/
https://ncbi.nlm.nih.gov/pubmed/17406529
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2006.229
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