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Using circular dichroism collected as a function of temperature to determine the thermodynamics of protein unfolding and binding interactions
Circular dichroism (CD) is an excellent spectroscopic technique for following the unfolding and folding of proteins as a function of temperature. One of its principal applications is to determine the effects of mutations and ligands on protein and polypeptide stability If the change in CD as a funct...
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2006
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| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2752288/ https://ncbi.nlm.nih.gov/pubmed/17406506 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2006.204 |
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