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Monitoring protein aggregation during thermal unfolding in circular dichroism experiments

Thermal unfolding monitored by spectroscopy or calorimetry is widely used to determine protein stability. Equilibrium thermodynamic analysis of such unfolding is often hampered by its irreversibility, which usually results from aggregation of thermally denatured protein. In addition, heat-induced pr...

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Detalhes bibliográficos
Main Authors: Benjwal, Sangeeta, Verma, Shikha, Röhm, Klaus-Heinrich, Gursky, Olga
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2249783/
https://ncbi.nlm.nih.gov/pubmed/16452626
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.051917406
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