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Dodine as a transparent protein denaturant for circular dichroism and infrared studies

The fungicide dodine combines the cooperative denaturation properties of guanidine with the mM denaturation activity of SDS. It was previously tested only on two small model proteins. Here we show that it can be used as a chemical denaturant for phosphoglycerate kinase (PGK), a much larger two‐domai...

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Detalhes bibliográficos
Publicado no:Protein Sci
Main Authors: Guin, Drishti, Sye, Kori, Dave, Kapil, Gruebele, Martin
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley and Sons Inc. 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4838651/
https://ncbi.nlm.nih.gov/pubmed/26941080
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.2914
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