Structure-Based Rational Design of a Phosphotriesterase

In silico substrate docking of both stereoisomers of the pesticide chlorfenvinphos (CVP) in the phosphotriesterase from Agrobacterium radiobacter identified two residues (F131 and W132) that prevent productive substrate binding and cause stereospecificity. A variant (W131H/F132A) was designed that e...

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Bibliographic Details
Main Authors: Jackson, Colin J., Weir, Kahli, Herlt, Anthony, Khurana, Jeevan, Sutherland, Tara D., Horne, Irene, Easton, Christopher, Russell, Robyn J., Scott, Colin, Oakeshott, John G.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 2009
Subjects:
Enzymology and Protein Engineering
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2725513/
https://ncbi.nlm.nih.gov/pubmed/19502439
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00629-09
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