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Millisecond phase kinetic analysis of elongation catalyzed by human, yeast and Escherichia coli RNA polymerase
Strategies for assembly and analysis of human, yeast and bacterial RNA polymerase elongation complexes are described, and methods are shown for millisecond phase kinetic analyses of elongation using rapid chemical quench flow. Human, yeast, and bacterial RNA polymerases function very similarly in NT...
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| Main Authors: | , , , , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2009
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2721912/ https://ncbi.nlm.nih.gov/pubmed/19398005 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2009.04.008 |
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