Millisecond phase kinetic analysis of elongation catalyzed by human, yeast and Escherichia coli RNA polymerase

Strategies for assembly and analysis of human, yeast and bacterial RNA polymerase elongation complexes are described, and methods are shown for millisecond phase kinetic analyses of elongation using rapid chemical quench flow. Human, yeast, and bacterial RNA polymerases function very similarly in NT...

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Main Authors: Kireeva, Maria, Nedialkov, Yuri A., Gong, Xue Qian, Zhang, Chunfen, Xiong, Yalin, Moon, Woo, Burton, Zachary F., Kashlev, Mikhail
Formato: Artigo
Idioma:Inglês
Publicado: 2009
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2721912/
https://ncbi.nlm.nih.gov/pubmed/19398005
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2009.04.008
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