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Fluorescently labeled ribosomes as a tool for analyzing antibiotic binding

Measuring the binding of antibiotics and other small-molecular-weight ligands to the 2.5 MDa ribosome often presents formidable challenges. Here, we describe a general method for studying binding of ligands to ribosomes that carry a site-specific fluorescent label covalently attached to one of the r...

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Detaylı Bibliyografya
Asıl Yazarlar: Llano-Sotelo, Beatriz, Hickerson, Robyn P., Lancaster, Laura, Noller, Harry F., Mankin, Alexander S.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Cold Spring Harbor Laboratory Press 2009
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC2714759/
https://ncbi.nlm.nih.gov/pubmed/19553343
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.1681609
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