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Fluorescently labeled ribosomes as a tool for analyzing antibiotic binding
Measuring the binding of antibiotics and other small-molecular-weight ligands to the 2.5 MDa ribosome often presents formidable challenges. Here, we describe a general method for studying binding of ligands to ribosomes that carry a site-specific fluorescent label covalently attached to one of the r...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Cold Spring Harbor Laboratory Press
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2714759/ https://ncbi.nlm.nih.gov/pubmed/19553343 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.1681609 |
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