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Ribosome binding by tRNAs with fluorescent labeled 3' termini.
Yeast and E. coli tRNAPhe samples were oxidized and labeled at the 3' end with dansyl hydrazine or fluorescein thiosemicarbazide. These tRNAs can bind to poly(U)-programmed E. coli 70S tight couple ribosomes in 25 mM magnesium at 8 degrees C. Two binding sites with binding constants of about 1...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1980
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC324366/ https://ncbi.nlm.nih.gov/pubmed/6160468 |
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