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Synthesis and functional activity of tRNAs labeled with fluorescent hydrazides in the D-loop
We describe an optimized procedure for replacing the dihydrouridine residues of charged tRNAs with Cy3 and Cy5 dyes linked to a hydrazide group, and demonstrate that the labeled molecules are functional in ribosomal activities including 30S initiation complex formation, EF–Tu-dependent binding to th...
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| Asıl Yazarlar: | , , |
|---|---|
| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
Cold Spring Harbor Laboratory Press
2009
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| Konular: | |
| Online Erişim: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2648706/ https://ncbi.nlm.nih.gov/pubmed/19118261 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.1257509 |
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