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A quantitative stopped-flow fluorescence assay for measuring polymerase elongation rates
The measurement of nucleic acid polymerase elongation rates is often done via a lengthy experimental process involving radiolabeled substrates, quenched elongation experiments, electrophoretic product separation, and band quantitation. In this work we describe an alternative real-time stopped-flow a...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2713312/ https://ncbi.nlm.nih.gov/pubmed/19406094 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2009.04.035 |
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