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A Fluorescence Polarization Based Screening Assay for Nucleic Acid Polymerase Elongation Activity
We have devised a simple high throughput screening compatible fluorescence polarization based assay that can be used to detect the elongation activity of nucleic acid polymerase enzymes. The assay uses a 5’ end labeled template strand and relies on an increase in the polarization signal from the flu...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2713175/ https://ncbi.nlm.nih.gov/pubmed/17475199 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2007.03.039 |
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