Fluorescence Polarization in Homogeneous Nucleic Acid Analysis

A new method for DNA diagnostics based on template-directed primer extension and detection by fluorescence polarization is described. In this method, amplified genomic DNA containing a polymorphic locus is incubated with oligonucleotide primers (designed to hybridize to the DNA template adjacent to...

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Bibliographic Details
Main Authors: Chen, Xiangning, Levine, Leanna, Kwok, Pui-Yan
Format: Artigo
Language:Inglês
Published: Cold Spring Harbor Laboratory Press 1999
Subjects:
Methods
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC310763/
https://ncbi.nlm.nih.gov/pubmed/10330129
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