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Human recombinant soluble guanylyl cyclase: Expression, purification, and regulation

The α1- and β1-subunits of human soluble guanylate cyclase (sGC) were coexpressed in the Sf9 cells/baculovirus system. In addition to the native enzyme, constructs with hexahistidine tag at the amino and carboxyl termini of each subunit were coexpressed. This permitted the rapid and efficient purifi...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Lee, Yu-Chen, Martin, Emil, Murad, Ferid
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: The National Academy of Sciences 2000
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC27097/
https://ncbi.nlm.nih.gov/pubmed/10995472
Tagiau: Ychwanegu Tag
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