Human recombinant soluble guanylyl cyclase: Expression, purification, and regulation
The α1- and β1-subunits of human soluble guanylate cyclase (sGC) were coexpressed in the Sf9 cells/baculovirus system. In addition to the native enzyme, constructs with hexahistidine tag at the amino and carboxyl termini of each subunit were coexpressed. This permitted the rapid and efficient purifi...
Gorde:
| Argitaratua izan da: | Proc Natl Acad Sci U S A |
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| Egile Nagusiak: | , , |
| Formatua: | Artigo |
| Hizkuntza: | Inglês |
| Argitaratua: |
National Academy of Sciences
2000
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| Gaiak: | |
| Sarrera elektronikoa: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC27097/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10995472/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.190333697 |
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