Human recombinant soluble guanylyl cyclase: Expression, purification, and regulation
The α1- and β1-subunits of human soluble guanylate cyclase (sGC) were coexpressed in the Sf9 cells/baculovirus system. In addition to the native enzyme, constructs with hexahistidine tag at the amino and carboxyl termini of each subunit were coexpressed. This permitted the rapid and efficient purifi...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2000
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC27097/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10995472/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.190333697 |
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