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Human mevalonate diphosphate decarboxylase: Characterization, investigation of the mevalonate diphosphate binding site, and crystal structure

Expression in E. coli of his-tagged human mevalonate diphosphate decarboxylase (hMDD) has expedited enzyme isolation, characterization, functional investigation of the mevalonate diphosphate binding site, and crystal structure determination (2.4 Å resolution). hMDD exhibits Vmax = 6.1 ± 0.5 U/mg; Km...

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Detalhes bibliográficos
Main Authors: Voynova, Natalia E., Fu, Zhuji, Battaile, Kevin P., Herdendorf, Timothy J., Kim, Jung-Ja P., Miziorko, Henry M.
Formato: Artigo
Idioma:Inglês
Publicado em: 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2709241/
https://ncbi.nlm.nih.gov/pubmed/18823933
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.abb.2008.08.024
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