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Cloning, overproduction, purification, crystallization and preliminary X-ray diffraction analysis of yeast glutaredoxin Grx5

Grx5 from the yeast Saccharomyces cerevisiae is a monothiol glutaredoxin that is involved in iron–sulfur cluster biogenesis. Here, yeast Grx5 was cloned and overproduced in Escherichia coli. The purified protein was crystallized using the hanging-drop vapour-diffusion method. Diffraction data for Gr...

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Bibliografske podrobnosti
Main Authors: Wang, Yi, He, Yong-Xing, Yu, Jiang, Zhou, Cong-Zhao
Format: Artigo
Jezik:Inglês
Izdano: International Union of Crystallography 2009
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2688435/
https://ncbi.nlm.nih.gov/pubmed/19478456
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309109018417
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