Overproduction, purification, crystallization and preliminary X-ray diffraction analysis of Trypanosoma brucei gambiense glycerol kinase

In the bloodstream forms of human trypanosomes, glycerol kinase (GK; EC 2.7.1.30) is one of the nine glycosomally compartmentalized enzymes that are essential for energy metabolism. In this study, a recombinant Trypanosoma brucei gambiense GK (rTbgGK) with an N-terminal cleavable His(6) tag was over...

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Detalhes bibliográficos
Main Authors: Balogun, Emmanuel Oluwadare, Inaoka, Daniel Ken, Kido, Yasutoshi, Shiba, Tomoo, Nara, Takeshi, Aoki, Takashi, Honma, Teruki, Tanaka, Akiko, Inoue, Masayuki, Matsuoka, Shigeru, Michels, Paul A. M., Harada, Shigeharu, Kita, Kiyoshi
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2010
Assuntos:
Crystallization Communications
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2833043/
https://ncbi.nlm.nih.gov/pubmed/20208167
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110000369
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