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Overproduction, purification, crystallization and preliminary X-ray diffraction studies of the human transcription repressor ERH

The human gene coding for the enhancer of rudimentary homologue (ERH) protein was overexpressed in Escherichia coli. The ERH protein was purified by anion-exchange, hydrophobic interaction and gel-filtration chromatography. Well diffracting single crystals were obtained by the vapour-diffusion metho...

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Detalhes bibliográficos
Main Authors: Jin, Tengchuan, Howard, Andrew, Golemis, Erica A., Wang, Yingtong, Zhang, Yu-Zhu
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1952306/
https://ncbi.nlm.nih.gov/pubmed/16511088
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309105012388
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