Comparison of the 5′ nuclease activities of Taq DNA polymerase and its isolated nuclease domain

Many eubacterial DNA polymerases are bifunctional molecules having both polymerization (P) and 5′ nuclease (N) activities, which are contained in separable domains. We previously showed that the DNA polymerase I of Thermus aquaticus (TaqNP) endonucleolytically cleaves DNA substrates, releasing unpai...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Lyamichev, Victor, Brow, Mary Ann D., Varvel, Virgil E., Dahlberg, James E.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1999
Assuntos:	Biological Sciences
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC26849/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10339555/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.96.11.6143
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