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Comparison of the 5′ nuclease activities of Taq DNA polymerase and its isolated nuclease domain

Many eubacterial DNA polymerases are bifunctional molecules having both polymerization (P) and 5′ nuclease (N) activities, which are contained in separable domains. We previously showed that the DNA polymerase I of Thermus aquaticus (TaqNP) endonucleolytically cleaves DNA substrates, releasing unpai...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Lyamichev, Victor, Brow, Mary Ann D., Varvel, Virgil E., Dahlberg, James E.
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1999
Fag:
Biological Sciences
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC26849/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10339555/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.96.11.6143
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