Use of Sloppy Molecular Beacon Probes for Identification of Mycobacterial Species

We report here the use of novel “sloppy” molecular beacon probes in homogeneous PCR screening assays in which thermal denaturation of the resulting probe-amplicon hybrids provides a characteristic set of amplicon melting temperature (T(m)) values that identify which species is present in a sample. S...

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Bibliographic Details
Main Authors: El-Hajj, Hiyam H., Marras, Salvatore A. E., Tyagi, Sanjay, Shashkina, Elena, Kamboj, Mini, Kiehn, Timothy E., Glickman, Michael S., Kramer, Fred Russell, Alland, David
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 2009
Subjects:
Mycobacteriology and Aerobic Actinomycetes
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2668336/
https://ncbi.nlm.nih.gov/pubmed/19171684
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.02043-08
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