Use of Sloppy Molecular Beacon Probes for Identification of Mycobacterial Species

We report here the use of novel “sloppy” molecular beacon probes in homogeneous PCR screening assays in which thermal denaturation of the resulting probe-amplicon hybrids provides a characteristic set of amplicon melting temperature (T(m)) values that identify which species is present in a sample. S...

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Detalhes bibliográficos
Main Authors: El-Hajj, Hiyam H., Marras, Salvatore A. E., Tyagi, Sanjay, Shashkina, Elena, Kamboj, Mini, Kiehn, Timothy E., Glickman, Michael S., Kramer, Fred Russell, Alland, David
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2009
Assuntos:
Mycobacteriology and Aerobic Actinomycetes
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2668336/
https://ncbi.nlm.nih.gov/pubmed/19171684
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.02043-08
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