Rapid, High-Throughput Detection of Rifampin Resistance and Heteroresistance in Mycobacterium tuberculosis by Use of Sloppy Molecular Beacon Melting Temperature Coding

Rifampin resistance in Mycobacterium tuberculosis is largely determined by mutations in an 80-bp rifampin resistance determining region (RRDR) of the rpoB gene. We developed a rapid single-well PCR assay to identify RRDR mutations. The assay uses sloppy molecular beacons to probe an asymmetric PCR o...

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Hlavní autoři: Chakravorty, Soumitesh, Kothari, Harsheel, Aladegbami, Bola, Cho, Eun Jin, Lee, Jong Seok, Roh, Sandy S., Kim, Hyunchul, Kwak, Hyungkyung, Lee, Eun Gae, Hwang, Soo Hee, Banada, Padmapriya P., Safi, Hassan, Via, Laura E., Cho, Sang-Nae, Barry, Clifton E., Alland, David
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology 2012
Témata:
Mycobacteriology and Aerobic Actinomycetes
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3405605/
https://ncbi.nlm.nih.gov/pubmed/22535987
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00143-12
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