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Mechanism of Dihydrouridine Synthase 2 from Yeast and the Importance of Modifications for Efficient tRNA Reduction

Dihydrouridine synthases (DUSs) are flavin-dependent enzymes that catalyze site-specific reduction of uracils in tRNAs. The mechanism of DUS 2 from Saccharomyces cerevisiae was studied. Previously published turnover rates for this DUS were very low. Our studies show that the catalytic cycle consists...

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Detalhes bibliográficos
Main Authors: Rider, Lance W., Ottosen, Mette B., Gattis, Samuel G., Palfey, Bruce A.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2667719/
https://ncbi.nlm.nih.gov/pubmed/19139092
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M806137200
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