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Allosteric transitions in cytochrome P450eryF explored with pressure-perturbation spectroscopy, lifetime FRET, and a novel fluorescent substrate, Fluorol-7GA
To establish a direct method to monitor substrate binding in cytochrome P450eryF applicable at elevated hydrostatic pressures we introduce a laser dye Fluorol-7GA (F7GA) as a novel fluorescent ligand. The high intensity of fluorescence and reasonable resolution of the excitation band from the absorb...
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| Hoofdauteurs: | , , |
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| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
2008
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| Onderwerpen: | |
| Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2662524/ https://ncbi.nlm.nih.gov/pubmed/18831537 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi8011803 |
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