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A method for detecting and preventing negative RNA interference in preparation of lentiviral vectors for siRNA delivery
The lentiviral vector is a useful tool for delivery of hairpin siRNA (shRNA) into mammalian cells. However, the efficiency of this system for carrying double-stranded siRNA (dsRNA) has not been explored. In this study we cloned the two forms of siRNA-coding sequence, a palindromic DNA with a spacer...
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| Autors principals: | , , , , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Cold Spring Harbor Laboratory Press
2009
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2661825/ https://ncbi.nlm.nih.gov/pubmed/19244361 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.985209 |
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