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A method for detecting and preventing negative RNA interference in preparation of lentiviral vectors for siRNA delivery

The lentiviral vector is a useful tool for delivery of hairpin siRNA (shRNA) into mammalian cells. However, the efficiency of this system for carrying double-stranded siRNA (dsRNA) has not been explored. In this study we cloned the two forms of siRNA-coding sequence, a palindromic DNA with a spacer...

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Detalhes bibliográficos
Main Authors: Zhou, Demin, Zhang, Jing, Wang, Cuiying, Bliesath, Joshua R., He, Qiuchen, Yu, Dehua, Li-He, Zhang, Wong-Staal, Flossie
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2661825/
https://ncbi.nlm.nih.gov/pubmed/19244361
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.985209
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