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A simplified miRNA-based gene silencing method for Drosophila melanogaster

MicroRNA-based RNA interference is commonly used to produce loss-of-function phenotypes in mammalian systems, but is used only sparingly in invertebrates such as C. elegans and D. melanogaster. Here, we evaluate this method in transgenic strains of D. melanogaster and cultured S2 cells. High through...

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Detalhes bibliográficos
Main Authors: Haley, Benjamin, Hendrix, David, Trang, Vinh, Levine, Michael
Formato: Artigo
Idioma:Inglês
Publicado em: 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2661819/
https://ncbi.nlm.nih.gov/pubmed/18598689
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ydbio.2008.06.015
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