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A New Approach for Characterizing the Intermediate Feature of α-Chymotrypsin Refolding by Hydrophobic Interaction Chromatography

A new approach for characterizing the intermediate of urea-denatured α-chymotrypsin (α-Chy) by hydrophobic interaction chromatography (HIC) is presented. The contact surface region (Z, S), affinity (logI), and the character of interaction force (j) of the α-Chy to the stationary phase of HIC (STHIC)...

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Detalhes bibliográficos
Main Authors: Ke, Congyu, Li, Jianjun, Liu, Zhenling, Geng, Xindu
Formato: Artigo
Idioma:Inglês
Publicado em: Molecular Diversity Preservation International (MDPI) 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2660661/
https://ncbi.nlm.nih.gov/pubmed/19333424
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms10020616
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