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EcI5, a group IIB intron with high retrohoming frequency: DNA target site recognition and use in gene targeting

We find that group II intron EcI5, a subclass CL/IIB1 intron from an Escherichia coli virulence plasmid, is highly active in retrohoming in E. coli. Both full-length EcI5 and an EcI5-ΔORF intron with the intron-encoded protein expressed separately from the same donor plasmid retrohome into a recipie...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Zhuang, Fanglei, Karberg, Michael, Perutka, Jiri, Lambowitz, Alan M.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Cold Spring Harbor Laboratory Press 2009
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2657007/
https://ncbi.nlm.nih.gov/pubmed/19155322
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.1378909
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