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EcI5, a group IIB intron with high retrohoming frequency: DNA target site recognition and use in gene targeting
We find that group II intron EcI5, a subclass CL/IIB1 intron from an Escherichia coli virulence plasmid, is highly active in retrohoming in E. coli. Both full-length EcI5 and an EcI5-ΔORF intron with the intron-encoded protein expressed separately from the same donor plasmid retrohome into a recipie...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Cold Spring Harbor Laboratory Press
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2657007/ https://ncbi.nlm.nih.gov/pubmed/19155322 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.1378909 |
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