Nalaganje...
Establishing wild-type levels of catalytic activity on natural and artificial (βα)(8)-barrel protein scaffolds
The generation of high levels of new catalytic activities on natural and artificial protein scaffolds is a major goal of enzyme engineering. Here, we used random mutagenesis and selection in vivo to establish a sugar isomerisation reaction on both a natural (βα)(8)-barrel enzyme and a catalytically...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
National Academy of Sciences
2009
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2656144/ https://ncbi.nlm.nih.gov/pubmed/19237570 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0810342106 |
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