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Purification of sputum samples through sucrose improves detection of Mycobacterium tuberculosis by polymerase chain reaction.

A method is described for the routine preparation of sputum samples for detection of Mycobacterium tuberculosis by using polymerase chain reaction amplification. Liquefaction of sputum samples with NaOH and subsequent removal of inhibitors of the polymerase reaction with a 50% sucrose centrifugation...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:J Clin Microbiol
Egile Nagusiak: Victor, T, du Toit, R, van Helden, P D
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: American Society for Microbiology (ASM) 1992
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC265320/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1624570/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.30.6.1514-1517.1992
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